通过频域重叠提升结构光照明显微的帧率，实现3倍提速。
Temporal resolution enhancement in Structured Illumination Microscopy using cascaded reconstruction
- 利用傅里叶域中折叠频谱的重叠区域进行级联重建。
- 在不改变硬件前提下实现最高3倍的帧率提升。
- 适用于现有SIM系统，简化光学设计且兼容活细胞成像。
结构光照明显微（SIM）通过将高频成分折叠至光路基带，实现超越衍射极限的空间信息获取。借助各类算法，可重建出包含亚波长信息的图像。尽管线性SIM在活细胞成像兼容性和光学装置简便性上优于其他超分辨方法，但其固有的时间分辨率受限于每幅图像需多帧采集。本文提出一种实用高效的重建方法，利用傅里叶域中折叠频谱成分的重叠区域，支持高达3倍的时间分辨率提升。该方法可直接应用于已有SIM实现，既提高时间分辨率，又简化光学装置。
原文摘要 · Abstract (English)
Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing sub-wavelength information can be reconstructed. While linear SIM is considered superior to other Super-Resolution methods in its compatibility with live cell imaging and optical setup simplicity, it is inherently limited in terms of its temporal resolution as each image requires multiple frames. Here we present a practical and efficient reconstruction approach supporting up to 3-fold temporal resolution increase with SIM, using overlapping regions in the folded frequency components within the Fourier domain. Our approach can be readily implemented in any previously introduced SIM realization to both improve the temporal resolution and to simplify the optical apparatus.
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