arXiv:2410.15229cs.CVcs.LG2024-10被引 3

用一张模糊照片快速判断细菌是否在群游,准确率超97%。

Deep Learning-based Detection of Bacterial Swarm Motion Using a Single Image

  • 基于深度学习,仅凭单张图像识别细菌群游行为。
  • 对SM3菌株检测灵敏度达97.44%,特异性100%。
  • 跨物种通用性强,适合手机端快速筛查。

区分细菌的群游与游动两种运动形式,在理论和临床层面均具重要意义。群游型细菌常与感染性疾病致病性相关,并可能具有治疗潜力。本文提出一种基于深度学习的群游分类器,仅需一张模糊图像即可快速、自动预测群游概率。相比传统依赖视频分析和人工处理的方法,该方法更适用于高通量场景,提供客观量化评估。分类器在Enterobacter sp. SM3数据上训练,盲测时对SM3新样本达到97.44%灵敏度和100%特异性。进一步测试显示,对未见菌种如Serratia marcescens DB10和Citrobacter koseri H6,分别实现97.92%灵敏度/96.77%特异性和100%灵敏度/97.22%特异性。该性能表明其可拓展至便携设备甚至智能手机,实现细菌群游运动的快速、客观、现场筛查,有助于炎症性肠病(IBD)和尿路感染(UTI)等疾病的早期发现与疗效评估。

原文摘要 · Abstract (English)

Distinguishing between swarming and swimming, the two principal forms of bacterial movement, holds significant conceptual and clinical relevance. This is because bacteria that exhibit swarming capabilities often possess unique properties crucial to the pathogenesis of infectious diseases and may also have therapeutic potential. Here, we report a deep learning-based swarming classifier that rapidly and autonomously predicts swarming probability using a single blurry image. Compared with traditional video-based, manually-processed approaches, our method is particularly suited for high-throughput environments and provides objective, quantitative assessments of swarming probability. The swarming classifier demonstrated in our work was trained on Enterobacter sp. SM3 and showed good performance when blindly tested on new swarming (positive) and swimming (negative) test images of SM3, achieving a sensitivity of 97.44% and a specificity of 100%. Furthermore, this classifier demonstrated robust external generalization capabilities when applied to unseen bacterial species, such as Serratia marcescens DB10 and Citrobacter koseri H6. It blindly achieved a sensitivity of 97.92% and a specificity of 96.77% for DB10, and a sensitivity of 100% and a specificity of 97.22% for H6. This competitive performance indicates the potential to adapt our approach for diagnostic applications through portable devices or even smartphones. This adaptation would facilitate rapid, objective, on-site screening for bacterial swarming motility, potentially enhancing the early detection and treatment assessment of various diseases, including inflammatory bowel diseases (IBD) and urinary tract infections (UTI).

细菌运动深度学习图像识别医学诊断

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