用注意力残差U-Net和视觉Transformer提升结核杆菌显微检测精度与效率

Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis, continues to be a major global health threat despite being preventable and curable. This burden is particularly high in low and middle income countries. Microscopy remains essential for diagnosing TB by enabling direct visualization of Mycobacterium tuberculosis in sputum smear samples, offering a cost effective approach for early detection and effective treatment. Given the labour-intensive nature of microscopy, automating the detection of bacilli in microscopic images is crucial to improve both the expediency and reliability of TB diagnosis. The current methodologies for detecting tuberculosis bacilli in bright field microscopic sputum smear images are hindered by limited automation capabilities, inconsistent segmentation quality, and constrained classification precision. This paper proposes a twostage deep learning methodology for tuberculosis bacilli detection, comprising bacilli segmentation followed by classification. In the initial phase, an advanced U-Net model employing attention blocks and residual connections is proposed to segment microscopic sputum smear images, enabling the extraction of Regions of Interest (ROIs). The extracted ROIs are then classified using a Vision Transformer, which we specifically customized as TBViT to enhance the precise detection of bacilli within the images. For the experiments, a newly developed dataset of microscopic sputum smear images derived from Ziehl-Neelsen-stained slides is used in conjunction with existing public datasets. The qualitative and quantitative evaluation of the experiments using various metrics demonstrates that the proposed model achieves significantly improved segmentation performance, higher classification accuracy, and a greater level of automation, surpassing existing methods.