用样品运动代替多图样，单次散斑照明实现超分辨成像
Sample Motion for Structured Illumination Fluorescence Microscopy
- 利用样品自身运动编码超分辨信息，仅需一个散斑图案
- 从多帧移动图像中联合估计运动轨迹与超分辨图像
- 适用于活体样本动态成像，降低硬件复杂度
结构光照明显微镜（SIM）通过不同照明模式采集多幅图像，计算重建突破衍射极限的分辨率。本文提出一种新方法：仅使用单一散斑照明图案，依靠样品自身的运动将超分辨信息编码至多幅原始荧光图像中。通过采集样品移动过程中的多帧图像，联合估计样品运动轨迹与超分辨图像。在仿真与实验中均验证了该方法的可行性。
原文摘要 · Abstract (English)
Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single speckle illumination pattern and relying on inherent sample motion to encode the super-resolved information in multiple raw images. From a set of raw fluorescence images captured as the sample moves, we jointly estimate both the sample motion and the super-resolved image. We demonstrate the feasibility of the proposed method both in simulation and in experiment.
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