C-FOCUS让双光子显微镜突破组织散射，实现小鼠脑内近毫米级成像。
Compressive Fourier-Domain Intensity Coupling (C-FOCUS) enables near-millimeter deep imaging in the intact mouse brain in vivo
- 结合傅里叶域调制与压缩感知，主动矫正散射
- 在活体小鼠脑中实现900微米以上高分辨成像
- 适合需要深层活体成像的神经科学研究
双光子显微镜是活体成像的强大工具，但因组织散射限制，成像深度通常仅数百微米，即使使用现有散射校正技术亦然。多数主动校正方法受光学记忆效应限制，仅适用于小区域。本文提出压缩式傅里叶域强度耦合（C-FOCUS），将傅里叶域强度调制与压缩感知结合，用于双光子显微镜的散射校正。利用C-FOCUS，我们实现了活体完整小鼠脑中YFP标记神经元和FITC标记血管在超过900微米深度的高分辨率成像。此外，成功实现通过完整成年小鼠颅骨进行跨颅成像，观察到YFP标记的树突结构。相较于未校正成像，1035纳米激发下荧光强度提升超20倍，实现了此前无法达到的高对比度荧光成像。C-FOCUS为快速、深层组织活体光学成像提供了通用策略。
原文摘要 · Abstract (English)
Two-photon microscopy is a powerful tool for in vivo imaging, but its imaging depth is typically limited to a few hundred microns due to tissue scattering, even with existing scattering correction techniques. Moreover, most active scattering correction methods are restricted to small regions by the optical memory effect. Here, we introduce compressive Fourier-domain intensity coupling for scattering correction (C-FOCUS), an active scattering correction approach that integrates Fourier-domain intensity modulation with compressive sensing for two-photon microscopy. Using C-FOCUS, we demonstrate high-resolution imaging of YFP-labeled neurons and FITC-labeled blood vessels at depths exceeding 900 um in the intact mouse brain in vivo. Furthermore, we achieve transcranial imaging of YFP-labeled dendritic structures through the intact adult mouse skull. C-FOCUS enables high-contrast fluorescence imaging at depths previously inaccessible using two-photon microscopy with 1035 nm excitation, enhancing fluorescence intensity by over 20-fold compared to uncorrected imaging. C-FOCUS provides a broadly applicable strategy for rapid, deep-tissue optical imaging in vivo.
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