开发首个牛体内连续甲烷监测系统，实现实时精准追踪反刍动物甲烷排放。

Enteric methane measurement from ruminant livestock faces fundamental trade-offs between accuracy and operational feasibility. Existing methods quantify methane after eructation and atmospheric dilution, limiting temporal resolution and confounding biological signals with environmental variables. We present SCOUT (Smart Cannula-mounted Optical Unit for Trace-methane), the first autonomous system for continuous in-vivo monitoring of ruminal headspace methane concentrations. The system addresses a critical engineering barrier through closed-loop gas recirculation that maintains anaerobic ruminal conditions during persistent headspace sampling. SCOUT was deployed on cannulated Simmental heifers under contrasting dietary treatments. Headspace concentrations were 100 to 1000 times higher than concurrent ambient sniffer readings, providing substantially greater signal resolution for characterizing methane dynamics. High-frequency monitoring revealed behavior-production coupling previously inaccessible, including rapid concentration changes ($14.5 \pm 11.3k$ ppm) associated with postural transitions within 15-minute intervals. Cross-platform comparison with ambient sniffers showed scale-dependent correspondence between production and release measurements, with an optimal correlation (r = -0.564) at 40-minute averaging windows consistent with eructation cycles. These results demonstrate that the rumen headspace contains continuous, biologically interpretable methane signals that SCOUT can reliably access, establishing the measurement infrastructure necessary for developing concentration-to-flux models that would support precision phenotyping, emission proxy calibration, and mitigation strategy evaluation.