用能量模型预测蛋白突变对结合能的影响，提升药物设计精度。

Predicting changes in binding free energy ($ΔΔG$) is a vital task in protein engineering and protein-protein interaction (PPI) engineering for drug discovery. Previous works have observed a high correlation between $ΔΔG$ and entropy, using probabilities of biologically important objects such as side chain angles and residue identities to estimate $ΔΔG$. However, estimating the full conformational distribution of a protein complex is generally considered intractable. In this work, we propose a new approach to $ΔΔG$ prediction that avoids this issue by instead leveraging energy-based models for estimating the probability of a complex's conformation. Specifically, we novelly decompose $ΔΔG$ into a sequence-based component estimated by an inverse folding model and a structure-based component estimated by an energy model. This decomposition is made tractable by assuming equilibrium between the bound and unbound states, allowing us to simplify the estimation of degeneracies associated with each state. Unlike previous deep learning-based methods, our method incorporates an energy-based physical inductive bias by connecting the often-used sequence log-odds ratio-based approach to $ΔΔG$ prediction with a new $ΔΔE$ term grounded in statistical mechanics. We demonstrate superiority over existing state-of-the-art structure and sequence-based deep learning methods in $ΔΔG$ prediction and antibody optimization against SARS-CoV-2.