仅用一次拉伸实验，就能精准反推心肌组织的力学参数和不确定性。

Cardiac muscle tissue exhibits highly non-linear hyperelastic and orthotropic material behavior during passive deformation. Traditional constitutive identification protocols therefore combine multiple loading modes and typically require multiple specimens and substantial handling. In soft living tissues, such protocols are challenged by inter- and intra-sample variability and by manipulation-induced alterations of mechanical response, which can bias inverse calibration. In this work we exploit spatially heterogeneous full-field kinematics as an information-rich alternative to multimodal testing. We recast EUCLID, an unsupervised method for the automated discovery of constitutive models, towards Bayesian parameter inference for highly nonlinear, orthotropic constitutive models. Using synthetic myocardial tissue slabs, we demonstrate that a single heterogeneous biaxial experiment, combined with sparse reaction-force measurements, enables robust recovery of Holzapfel-Ogden parameters with quantified uncertainty, across multiple noise levels. The inferred responses agree closely with ground-truth simulations and yield credible intervals that reflect the impact of measurement noise on orthotropic material model inference. Our work supports single-shot, uncertainty-aware characterization of nonlinear orthotropic material models from a single biaxial test, reducing sample demand and experimental manipulation.