arXiv:2511.17860eess.IV2025-11被引 2

用光纤板提升多色荧光成像的角稳定性,让微型无透镜传感器更灵敏且适用性强。

A Versatile Optical Frontend for Multicolor Fluorescence Imaging with Miniaturized Lensless Sensors

  • 用光纤板吸收斜向漏光,解决滤光片角度敏感问题。
  • 高NA设计使灵敏度提升59倍,分辨率仅下降3.2倍。
  • 支持三色荧光成像,1毫米工作距离下分辨率达110微米。

无透镜成像可实现极紧凑的荧光传感器,推动体内成像和低成本即时检测应用。此类传感器需滤光片阻挡激发光而通过荧光发射,但传统薄膜干涉滤光片对入射角敏感,难以用于无透镜系统。本文系统分析并优化了利用光纤板(FOP)吸收偏离轴线的漏光,同时提升图像分辨率的技术。仿真表明,FOP的数值孔径(NA)决定固有设计权衡:更高NA能快速提升收集效率,但会降低分辨率、增加器件厚度及荧光激发效率。我们优化了两种前端,全宽半最大值(FWHM)分别为8.3°和45.7°。实验显示,为抑制散射,需在FOP两侧均配置滤光片。使用520-μm厚的高NA设计,荧光灵敏度提高59倍,分辨率仅下降3.2倍;低NA设计则可在1毫米工作距离下实现110-μm分辨率的三色荧光成像。整体上,该光学前端具有高度灵活性,适用于多种荧光标记物、照明方式及无透镜成像技术。

原文摘要 · Abstract (English)

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing fluorescent emissions. However, conventional thin-film interference filters are sensitive to angle of incidence (AOI), complicating their use in lensless systems. Here we thoroughly analyze and optimize a technique using a fiber optic plate (FOP) to absorb off-axis light that would bleed through the interference filter while improving image resolution. Through simulations, we show that the numerical aperture (NA) of the FOP drives inherent design tradeoffs: collection efficiency improves rapidly with a higher NA, but at the cost of resolution, increased device thickness, and fluorescence excitation efficiency. To illustrate this, we optimize two optical frontends with full-width at half maximums (FWHMs) of 8.3° and 45.7°. Implementing these designs, we show that angle-insensitivity requires filters on both sides of the FOP, due to scattering. In imaging experiments, the 520-$μ$m-thick high-NA design is 59$\times$ more sensitive to fluorescence while only degrading resolution by 3.2$\times$. Alternatively, the low-NA design is capable of three-color fluorescence imaging with 110-$μ$m resolution at a 1-mm working distance. Overall, we demonstrate a versatile optical frontend that is adaptable to a range of applications using different fluorophores, illumination configurations, and lensless imaging techniques.

无透镜成像荧光检测光学前端多色成像

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