用计算方法分析雏鸡叫声，发现孕期接触药物会改变其发声模式。

In young animals like poultry chicks (Gallus gallus), vocalisations convey information about affective and behavioural states. Traditional approaches to vocalisation analysis, relying on manual annotation and predefined categories, introduce biases, limit scalability, and fail to capture the full complexity of vocal repertoires. We introduce a computational framework for the automated detection, acoustic feature extraction, and unsupervised learning of chick vocalisations. Applying this framework to a dataset of newly hatched chicks, we identified two primary vocal clusters. We then tested our computational framework on an independent dataset of chicks exposed during embryonic development to vehicle or Valproic Acid (VPA), a compound that disrupts neural development and is linked to autistic-like symptoms. Clustering analysis on the experimental dataset confirmed two primary vocal clusters and revealed systematic differences between groups. VPA-exposed chicks showed an altered repertoire, with a relative increase in softer calls. VPA differentially affected call clusters, modulating temporal, frequency, and energy domain features. Overall, VPA-exposed chicks produced vocalisations with shorter duration, reduced pitch variability, and modified energy profiles, with the strongest alterations observed in louder calls. This study provides a computational framework for analysing animal vocalisations, advancing knowledge of early-life communication in typical and atypical vocal development.