用AI虚拟鱼引导真实鱼群，实现实时闭环控制。

Guiding collective motion in biological groups is a fundamental challenge in understanding social interaction rules. In this study, we propose a deep reinforcement learning (RL) framework for closed-loop guidance of fish schools using virtual agents. These agents are controlled by policies trained via Proximal Policy Optimization (PPO) in simulation and deployed in physical experiments with rummy-nose tetras (Petitella bleheri), enabling real-time interaction between artificial agents and live individuals. To cope with the stochastic behavior of live individuals, we designed a composite reward function that balances directional guidance with cohesion, providing a form of functional biomimicry at the level of the control objective. Our systematic evaluation of visual parameters showed that a white background and larger stimulus sizes produced the highest guidance efficacy among the tested conditions in physical trials. Furthermore, evaluation across group sizes and agent configurations indicated that guidance efficacy decreased as the group size increased from five to eight individuals, and that using multiple independently controlled agents did not improve guidance. Analysis of agent motion in the physical trials indicated that, under the learned policy, the agent moved toward the target while remaining close to the school and re-approached the school after advancing too far ahead. This study highlights the potential of deep RL for closed-loop guidance of fish schools and identifies challenges in maintaining artificial influence in larger groups.