整合显著性与稳定性分析,高效识别适应性强的优良品种
Significance and Stability Analysis of Genotype-Environment Interaction using GxEStat
- 结合混合效应模型与多种稳定性方法,统一分析基因型-环境互作
- 可量化环境、基因型及互作效应的显著性,评估品种稳定性
- 提供交互式R工具,提升育种数据分析效率与可复现性
基因型-环境(GxE)互作会影响基因型在不同环境中的表现,降低育种中基因型评价与选择的可靠性。深入分析GxE互作对于理解遗传优势或缺陷在不同环境下的表达至关重要,并有助于识别优异且稳定的基因型。本研究提出一个集成计算框架,将显著性分析与稳定性评估整合于统一工作流中。该框架采用混合效应模型量化环境、基因型及GxE效应的显著性,并结合多种稳定性分析方法,刻画基因型适应性、环境代表性及跨环境表现一致性。为支持可复现的统计分析,开发了GxEStat交互式R平台,自动完成模型构建、参数估计、统计推断与图形可视化。该平台显著提升多环境试验分析的效率、可复现性与可及性。真实育种数据的应用验证了该框架在实际育种研究中的有效性。代码已开源:https://github.com/mason-ching/GxEStat。
原文摘要 · Abstract (English)
Genotype-environment (GxE) interactions can influence the performance of genotypes across diverse environments, limiting the reliability of genotype evaluation and selection in breeding programs. In-depth analysis of GxE interactions is therefore essential for understanding how genetic advantages or defects are expressed under varying environmental conditions and for identifying superior and stable genotypes. This study presents an integrated computational framework for GxE analysis that combines significance and stability evaluation within a unified analytical workflow. The framework incorporates the mixed effect modeling to quantify the significance of environment, genotype, and GxE effects, together with multiple stability analysis approaches for characterizing genotype adaptability, environmental representativeness, and performance consistency across environments. To support reproducible statistical analysis, we develop GxEStat, an interactive R platform that automates model construction, parameter estimation, statistical inference, and graphical visualization. GxEStat substantially improves analytical efficiency, reproducibility, and accessibility for multi-environment trial analysis. Applications to real breeding datasets demonstrate the effectiveness of the proposed framework for practical breeding research. Codes are available at https://github.com/mason-ching/GxEStat.
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