用50美元手机平台实现3D光学显微，适合教育与野外使用
Inexpensive Optical Projection Tomography on a Mobile Phone Platform
- 手机+廉价镜头+步进电机旋转，低成本构建光学投影断层成像系统
- 分辨率3.91μm，可清晰还原斑马鱼胚胎脊柱等三维结构
- 适合资源有限地区、教学或野外科研的便携式3D显微方案
本文提出一种基于智能手机平台的低成本光学投影断层成像（OPT）系统，用于三维光学显微。系统采用iPhone相机配合低价商用显微镜头、步进电机实现样品旋转、LED光源及自定义3D打印组件，总成本约50美元（不含手机）。为支持系统评估，开发了一种低成本斑马鱼仿体制造方法，将固定幼虫嵌入紫外固化树脂中。通过棋盘格标定相机，利用1951年空军分辨率靶标估算有效放大倍率。在样品旋转过程中采集投影图像，转换为衰减图像并校正光照不均匀性，每层采用滤波反投影法重建，最终堆叠生成3D体积。系统实现3.91μm分辨率，成功呈现斑马鱼仿体的脊柱等解剖结构。结果表明，基于手机的OPT可提供可及、便携且低成本的3D显微能力，适用于教育、野外工作及资源受限场景。
原文摘要 · Abstract (English)
This work presents an inexpensive optical projection tomography (OPT) system built on a mobile phone platform for three-dimensional optical microscopy. The system uses an iPhone camera together with a low-cost commercial microscope lens attachment, a stepper motor for sample rotation, LED illumination, and custom 3D-printed components, with a total component cost of approximately 50 US dollars excluding the phone. To support system evaluation, we also developed a low-cost method for fabricating a zebrafish phantom by embedding fixed larvae in UV-cured resin. Camera calibration was performed using a checkerboard target, and effective magnification was estimated with images of a 1951 Air Force resolution target. Projection images acquired during sample rotation were converted to attenuation images and corrected for field nonuniformity. Each slice was reconstructed with filtered backprojection and the resulting slices were stacked into a 3D volume. The completed system achieved a resolution of 3.91 $μm$ and produced volumetric reconstructions in which anatomical features of the zebrafish phantom, including the spine, were clearly visible. These results demonstrate that mobile-phone-based OPT can provide accessible, portable, and low-cost 3D microscopy, with potential utility for education, field work, and resource-limited settings.
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