用手机摄像头低成本检测微量荧光分子，替代昂贵的实验室设备。
Design and Validation of a Low-Cost Smartphone Based Fluorescence Detection Platform Compared with Conventional Microplate Readers
- 用手机相机配合96孔板，通过RGB颜色值推算荧光浓度。
- 在10^-7到10^-4 M范围内，检测精度与商用读数仪相当。
- 适合资源有限地区或现场快速检测，无需专业仪器。
本文设计并验证了一种基于智能手机的低成本荧光检测平台，用于在稀释样本中检测特定微生物和分子。采用适配常规96孔板的装置结构，使手机摄像头可作为光学探测器。与传统微孔板读数仪（如Perkin Elmer Victor Machine）相比，该系统不依赖昂贵的激发滤光片、阻挡滤光片和光电倍增管，仅需手机摄像头即可完成荧光信号采集。核心策略是建立样本图像在RGB色彩空间中的颜色值与荧光物质摩尔浓度之间的关系。实验表明，在10^-7至10^-4 M浓度范围内，该方法具有良好的线性响应与检测灵敏度，性能接近商用设备。本研究为资源受限环境下的即时检测提供了可行方案。
原文摘要 · Abstract (English)
A low cost fluorescence-based optical system is developed for detecting the presence of certain microorganisms and molecules within a diluted sample. A specifically designed device setup compatible with conventional 96 well plates is chosen to create an ideal environment in which a smart phone camera can be used as the optical detector. In comparison with conventional microplate reading machines such as Perkin Elmer Victor Machine, the device presented in this paper is not equipped with expensive elements such as exciter filer, barrier filter and photomultiplier; instead, a phone camera is all needed to detect fluorescence within the sample. The strategy being involved is to determine the relationship between the image color of the sample in RGB color space and the molar concentration of the fluorescence specimen in that sample. This manuscript is a preprint version of work related to a publication in IEEE. The final version may differ from this manuscript.
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