针对浮游生物识别中未知类导致的群落估算偏差，提出基于群落层面的评估与校准方法。

Automated plankton image recognition is increasingly used in aquatic ecosystem monitoring, but deployed classifiers inevitably encounter unseen taxa and non-target particles. Open-set recognition methods are usually evaluated with sample-level metrics such as AUROC, AUPR, and FPR@95% unknown-recall operating points, whereas ecological monitoring depends on community-level estimates of taxon abundance and diversity. This study examines the mismatch between these objectives using controlled pseudo-communities and three datasets spanning marine zooplankton imaged by ZooScan, marine phytoplankton imaged by IFCB, and freshwater plankton imaged by an in-situ camera. We define Open-Set Community Distortion (OSCD), a Bray-Curtis-style error over known taxa plus an unknown bin, with directional components distinguishing known-taxon overestimation from underestimation. Closed-set classifiers achieved high known-class accuracy, but unknown samples were often absorbed with high confidence and in structured ways. Sample-level OOD metrics were not sufficient to select ecological operating points: for MSP, FPR@95% unknown-recall thresholds produced large test-community OSCD on all three datasets mainly because true known taxa were over-rejected into the unknown bin. Community-aware threshold calibration reduced MSP OSCD relative to fixed 95% known recall on SYKE-ZooScan 2024 and SYKE-IFCB 2022; on ZooLake the fixed-recall baseline was already close to the community-aware threshold, and the best community-level method was a prototype-distance variant rather than MSP. The benefit of community-aware calibration therefore depends on validation-community representativeness and the gap between fixed recall and the community optimum. These results show that open-set plankton recognition should be evaluated as an ecological measurement problem, not only as a sample-level detection task.