用多层指纹识别AI网页代理，97%准确率区分其与人类和传统爬虫。

As AI web agents proliferate, combining large language models with autonomous, browser-level control, indiscriminate content scraping by web agents has emerged as a privacy and security challenge. Existing defenses, such as robots.txt and active bot-blocking, are insufficient, as they are widely violated and easily circumvented. In this work, we demonstrate that AI web agents can be effectively distinguished from humans and traditional crawlers using a multi-layer fingerprint based on both network layer characteristics (e.g., TLS, HTTP) and browser interaction behavior. We implement this mechanism as a programmatic logging framework that can be deployed on a live, instrumented domain. By analyzing six prominent agent frameworks (AutoGen, Browser Use, Claude, Gemini, Operator, and Skyvern), we uncover latent structural differences in how these systems assemble HTTP requests, establish TLS/HTTP connections, and execute autonomous browser actions. Feeding these multi-layer features into a decision tree classifier, our framework achieves high-fidelity identification (97% accuracy), successfully isolating distinct agent architectures and differentiating agent traffic from both human browsing baselines and legacy crawlers. Our findings demonstrate that cross-layer agent tracking provides a robust, evasion-resistant strategy for content protection and web security policy enforcement.