用牛奶红外光谱识别产奶母牛能量失衡分群，可早期预警健康风险。

Clustering methods have been used to identify distinct groups of milk samples, cows, or herds. Fourier-transform infrared (FTIR) spectroscopy, particularly mid-infrared (MIR) spectroscopy, has been applied to individual cow milk samples to predict various milk traits. Applying clustering directly to MIR spectral data may reveal latent groups of cows associated with milk traits or health disorders and can help prevent these conditions or monitor at-risk animals. This study aimed to identify groups of individual dairy cows in early lactation directly from milk MIR spectra and to analyze their associations with milk traits. Using a dataset of 407,632 individual milk MIR records from 3,408 commercial farms, we combined (i) spectral filtering that selects informative wavenumbers, (ii) two dimensionality-reduction methods: principal component analysis (PCA) and an autoencoder, and (iii) two clustering algorithms: k-means and spectral clustering to yield eight different clustering approaches. We regrouped the assigned clusters into meta-clusters that encompassed the most similar ones identified by the eight approaches. Our results revealed five distinct meta-clusters of early-lactation individual dairy cows significantly associated with milk traits. Despite substantial differences, the eight approaches converged on the same five meta-clusters, and the classic, computationally efficient PCA-based k-means approach using the full spectrum recaptured clusters identified by more sophisticated, computationally intensive approaches. The five meta-clusters were strongly associated with DIM and appeared to reflect a gradient of negative energy balance (NEB) severity: severe, moderate, and possibly mild, while the remaining two likely represented cows recovering from NEB, one with rapid restoration of energy balance and one in early recovery.