默认阈值导致酶分类预测严重失准，真实效果远低于表面准确率。

Automated prediction of Enzyme Commission (EC) numbers plays a central role in functional annotation and computational drug discovery. However, standard multi-label machine learning pipelines frequently rely on default decision thresholds (t=0.50), assuming balanced prior distributions across target heads. In this study, we present a systematic empirical diagnostic of uncalibrated fixed decision boundaries operating under severe class imbalance across N = 14,096 annotated compounds categorized into six primary EC classes (EC1-EC6). Our results highlight a pronounced Accuracy Paradox: while the multi-label system achieves a deceivingly high mean accuracy of 77.16%, the macro F1-score (0.3976) and macro recall (0.3872) reveal severe predictive breakdown. Majority target classes suffer from hyper-sensitivity and over-prediction, whereas minority classes exhibit sharp recall decay, culminating in a total decision boundary collapse for EC6 (Recall = 0.00%) despite underlying discriminative power (ROC-AUC = 0.5857). Feature correlation analysis further reveals high linear redundancy among topological indices relative to fingerprint density metrics. Ultimately, this diagnostic study demonstrates that standard point predictions mask critical errors in bioinformatics workflows. We establish target-specific threshold optimization and post-hoc conformal calibration as essential, open-source post-processing safeguards for reliable applied machine learning and deep learning architectures.